That’s a fascinating observation! I’d be curious about the viability of the egg sac as well. If the spider truly had no contact with a male after being collected, parthenogenesis would certainly be an interesting possibility to investigate. It might also be worth checking whether females can retain sperm from an earlier mating or whether there are documented cases in related Latrodectus species. Hopefully someone with experience breeding black widows can shed some light on this!
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