The way that I cultured the fruit flies was that I would be VERY conscientious about starting a new culture WHENEVER I started feeding off a culture. I would use those first flies from the culture. I heard that if you wait and use the flies from later on, they aren't as fertile. I also put a drop or two of honey into my cultures (supposed to keep down the smell). Another trick that I learned was to gently spray the new culture with water after putting the bakers' yeast in. I heard something about how it would make the yeast active? You might want to check the temps of the room where you are keeping the cultures. Too warm or too cold is not good, it affects their fertility. I found that (at least at my house) that they were affected by lack of light. I had to keep them out on a shelf. Remember that hydeii take a lot longer to culture than melagonaster. I heard that it takes twice as long for hydeii.
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phflame

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