the twitching and the limpness sounds like calcium deficiency, but by the looks of the pics thats probably not the case. but they do develop eggs, even when they are by themselves. how long was that peacock day gecko with her. day geckos are the most territorial little devils. i had two adult females in a quarentine tank that was like two gal. they were fine, but i separated them for a while. I later put them back together in a well set up ten gal before introducing a male and with in two days the one female was dead. that gecko looked pretty old though. was she significantly smaller when you got her? she could have just been real old and had some kind of blockage with her eggs.

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