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RE: How much venom can a shed fang hold?

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Posted by: phobos at Thu Mar 30 05:44:09 2006   [ Email Message ] [ Show All Posts by phobos ]  
   

Hi Wolfgang:

I should have posted my methodology along with the results. My schedule has been hellish the past week, so I just posted the results.

I had the fang clamped in a pair of hemostats to give the fang a stable purchase while I was injecting water from my adjustable micropipetor from my lab at work. The pipetor tip was narrow enough to fit tightly into the duct end of the fang.

I started with 10ul of water to get a feel for the approximate quantity that was needed to see water appear at the exit of the fang without forming a drop. I used this as a staring point because of your inital caculation was for a 20mm fang and this was only 10mm. I "Blew" the water remaining inside the fang out between trials with gas from a "dust off" can.

After five subsquent tries with the pipettor set a 9ul I was satisfied that this was the volumn the fang could contain without designing an "insane" experiment where I weighed the fang dry and wet several times on the nano-balance in the chemistry department at work. I was trying to keep this simple and accuracy beyond this was not required.

Cheers!

Al
-----
Confidence is what you feel before you comprehend the situation.


   

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