Unless you had a necropsy done on the chameleon after it died, there is no way to know what caused it's death. Twitching can be symtoms of MBD but it also can be due to dificiencies in other nutrients other than calcium and Vitamine D3. The only virus I know that has affected bearded dragons in the past is the adenovirus which is not very easy to detect. It can produce symtoms very much like early MDB, parasite infections etc. Only a liver biopsy or blood test for the virus may detect it. On a good note, I haven't heard anything further about bearded dragons contracting the adenovirus for a good year or more, though this doesn't mean it isn't around, may never have been diagnosed.

My suggestion is to take your bearded dragon to your vet right away, have his blood calcium/bone density checked and get a fecal done to see what could be causing the twitching. Blood work and other tests should be done (as needed) to test for any sort of nutrient deficiency or toxicity (too much of certain nutrients can be as bad if not worse than not enough).
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PHLdyPayne

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