I would recommend removing the plexigalss barrier and replacing it with a piece of plywood or some other material that the turtles cannot see through.

It is not unusual for box turtles to try to get through barriers that they can see through (this is one of the reasons why glass tanks are not considered to be suitable enclosures for them).

I don't think that there is any clear consensus on what the duration of quarantine should be, so I'm reluctant to tell you how many weeks/months you should keep your box turtles separate, but do consider this: a diseased box turtle can appear to be asymptomatic for a long time before anything starts to go wrong.

The longer the quarantine, the better. I've heard anything from 4 weeks to 18 months: hopefully some of the other posters here can make some suggestions.

I'll link you to an online article on the subject in a minute.

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