You do not need any substrate but you do need a egg chamber. Get yourself a egg container that fits inside your incubator and put two pieces of egg crate in the bottom. Fill the bottom of the container with water up to the first leval of egg crate and then seal the egg container with a lid. Always take a look at it every other day and open the lid for a few seconds to let the air exchange. At 89 degrees it should be about 56-60 days before hatching. I wrote this in a hurry if there is anything you did not understand just ask and I can post pics when I get home. Paul at Steel City Exotics

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