Hi Warren,

I have had at least 4-5 of these types of litters over the years and poss partho baby I kept died (usually during adulthood). I tried to breed a poss anery female a couple years ago and she never bred (and died the subsequent year, or later that same year). I am even sitting on a partho litter now--a couple were weak and died--and the rest are doing fine (at least for now). So needless to say, I am glad someone with resources, the experience/education, and facilities were able to study these frequent phenomena in "old" boids.



I have a couple questions about your paper. In Table 1 you list the various loci and micro-sat motifs, but what are the "sequences" you are listing? Are these variable regions within these loci? And, what are the "F" and "R"s? The rest of the table makes sense to me.



And to Table 2, are the numbers listed under each locus (e.g. 295/295) bp lengths and supposed to represent hetero- and/or homozygosity, by length?



And also, a question on this statement: "At this stage of development in the sexually produced males examined, both testes and ovaries were present...". Are you saying the similar aged males (to the lone female examined) had both sex organs? Maybe, I missed something or misinterpreted the sentence, or its connection elsewhere.



And lastly, you speculate the reason for WW females is the possibility the dam of the litter was hemizygous or was it some meiotic aberration during oogenesis (from the dam)?



I lied--here is another. There appears to be some variation in the offspring; can you speculate what is happening? There seems to be some kind of gene shuffling going on. I am not familiar with oogenesis in boids, or terminal fusion for that matter (w/second polar body). If meiosis is occurring, there should be some gene mixing, right?



Sorry, for all the questions. I am just trying to understand every aspect of this paper so I may explain it to my students.



Back to teaching...good work!



Here is a partho anery whom died two years ago as an adult:



-----
Ron Michelotti



Class Reptilia

www.classreptilia.com

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