Posted by:
creptilia
at Thu Nov 4 13:31:57 2010 [ Email Message ] [ Show All Posts by creptilia ]
Hi,
Thank you for taking the time to answer my questions. The tables make perfect sense (I realize the PCR information is important for the methods section). I am shocked to hear males have both sex organs at birth, or as neonates. I am hoping you can speak more to terminal fusion and oogenesis (in boas)--I am assuming oogenesis in boas is similar to humans. Also, how did you know the babies were WW females specifically (as opposed to ZW) if the sex chromosomes are homomorphic? What was the test? (I may have missed this part.)
Now to your questions:
1) How do you know your litters were parthenogenetic without genetic testing?
I don't—I was unaware of any sort of testing for boids and nor did I have the means. But, the answers to the following questions should confirm? my speculation.
2) What were teh circumstances surrounding each litter?
Off the top of my head (not good science)--all high prevalence of homozygotes and slugs:
1) Sharp albino x Anery= all female anerys (around 5) and high slug count; don't believe the Sharp was het anery based on further breeding trials (and the male albino was not sold as such)
2) Sharp Sunglow x Ghost= all female anerys (2-3) and 2 deformed super looking ghosts (around 5 animals in total) and high slug count; there is a possibility of het anery on the part of the male, but I doubt he sired the litter based on the outcome (and my previous experiences; also not good science)
3) VPI T positive x Ghost= 5 (or so) anerys and 2 (I think) super looking ghosts (all female) and high slug count (I have this data at home); male T pos had been bred to her before with no anery or ghost offspring (i.e. he is not het anery)
3) How many females produced these litters?
I believe four (an anery, 2-3 different ghosts); I should have kept better records. I can probably dig up more data from old records.
4) What were the ratios of offspring to slugs? Also were they all female litters. What were the litter sizes?
all female; 5 live (w/sometimes few stillborn deformities) to approx 15-20 slugs; the litters are all 5-7 live offspring
5) Are you willing to provide shed skins from each live animal, tissue from dead? babies, adult females, and any males those "parthenogenetic" litter producing female were with?
I only have the last litter, but YES! The parents from the first two aforementioned litters have been sold and the parthenogens have either died or been sold (not understanding what had happened). I have only recently begun to speculate what has been occurring over the last 3-5 years.
Sorry for the sloppy response, I am also trying to teach at the same time.
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Ron Michelotti
Class Reptilia
www.classreptilia.com
[ Hide Replies ]
- Parthenogenesis in Boa constrictors - Warren_Booth, Tue Nov 2 19:15:04 2010
- Great Article dr Booth easy for - LarM, Tue Nov 2 19:41:28 2010
- Nice Warren!......... - RuBeN14, Tue Nov 2 20:15:46 2010
- New Scientist write-up - Warren_Booth, Tue Nov 2 20:52:46 2010
- RE: Parthenogenesis in Boa constrictors - boawoman, Wed Nov 3 00:28:01 2010
- More pics - boawoman, Wed Nov 3 00:32:05 2010
- Link to the actual paper - Warren_Booth, Wed Nov 3 07:00:55 2010
- Discovery News write-up - Warren_Booth, Wed Nov 3 07:03:15 2010
- Great article Dr. Booth - ajfreptiles, Wed Nov 3 09:28:27 2010
- RE: Parthenogenesis in Boa constrictors - ALT, Wed Nov 3 10:25:36 2010
- Questions... - creptilia, Wed Nov 3 15:50:31 2010
- RE: Parthenogenesis in Boa constrictors - kblumenthal, Fri Nov 5 12:12:14 2010
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RE: Questions... - creptilia, Thu Nov 4 13:31:57 2010