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Help - any experienced microscope users?

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Posted by: dwherp at Mon Mar 5 12:47:57 2012   [ Email Message ] [ Show All Posts by dwherp ]  
   

Hello,
I'm looking for assistance from anyone who knows how to collect a ball python sperm sample to be viewed under a microscope... and yes, I'm expecting some good humor to come from this post, but I am seriously looking for help here. I recently obtained a lab quality microscope for the primary purpose of using it to tell when a male is getting the job done (i.e. depositing adequate quantities of live sperm). I've seen videos in the past of snake sperm under the scope, so I know it can be done, but so far I'm not having much luck... I'm sure my technique is just all wrong. So far I've tried swabbing the cloaca of a recently bred female with a wet Qtip and then wiping it on slide. I've also tried to get a sample using a large sexing probe, but so far nothing has resulted in being able to see any swimmers.
Any help is much appreciated!
Dan


   

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