For me, the number one cause of egg loss during incubation is from imperfectly formed eggs. These eggs are fertile at laying but have one or more weak spots on the eggs. The weak spots are noticeable as sections of the egg where the color isn't as bright white as the rest. This color may vary from off-white or grayish, to yellow or brown.
Two things can happen to these eggs. The first problem occurs because on a moist incubation medium like I use, the eggs absorb water and become firm with an internal pressure as a result. If the weak spots are significant in size, or the shell is very thin there, the egg will simply blow open (leak). I have never had an egg I could save after it blew open (although Dean claims he saved one once by using electrical tape to wrap the egg).
Even if the eggs don't fail in this manner, the weak spots on the shell are places where mold can penetrate easier. If I find mold on an egg, I dust the egg there with dry 50% benomyl (available as a nursery item for plants). This has saved a lot of eggs for me and appears to have no effect on the developing hatchling.
I have wanted to try experimenting with proactively dusting the eggs at weak spots, and I will try this next year. It is better to prevent the mold from starting rather than to try to kill it after it has started, in my opinion.
I have experimented with spray miconazole applied to brushes, and allowed to dry before brushing the weak spots (spray miconazole has alcohol in it which might hurt the egg). In my hands, benomyl works significantly better.
Benomyl is extremely toxic to fish, but is relatively safe with most birds, and with snake eggs as I mentioned. Are snakes more related to birds or to fish? If you don't know, you should be able to guess from these results.
Robert.