One of the members asked if the female needs to mate again after laying the first clutch in order to get fertile eggs in a second clutch produced that season (double clutch). I can now state for certain that an Eastern Black Rat can produce fertile eggs in the second clutch even without mating after the second clutch. I am certain of this because my snake's second clutch has started to pip. I am not saying that your chances aren't better if the female is mated again after the first clutch, but I can now say for a fact that your female can produce fertile eggs without being reintroduced to the male.

