The pet store I frequent on the south end of the Grand Rapids area (golfdiva might know of V.I. Pets on S. Division in the Cutlerville area) says to used dechlorinated (aged) water with aquatic turtles. They say that when the tank is low, adding water fresh from the tap will cause huge fluctuations in bacteria and algal growth, and it is best to used aged water to prevent these fluctuations.
You faolks on the box turtle web forum mostly seem to use aged water too.
But in Jim Harding's book Michigan Turtles and Lizards he says tap is fine, no mention of aging/dechlorinating the water. In the research papers I have read of aquatic turtles, they usually say they use straight (unaged) tap water. And at John Ball Zoo, the the aquatic turtles get fresh tap water (with chlorine) every day.
I suppose it depends on how often one changes the water. In the research papers I mention, I think the authors usually said that water is changed twice per week. In JBZ, the wood and spotted turtles' water gets changed every day. So, in both situations, not enough time is allowed to build up bacteria or algae it would seem, hence, no need to worry about fluctuations.
One group of animals at the zoo DOES get aged water - amphibians.
But I really suspect that chlorine is of no harm to box turtles.

