well if you have 4 frogs and 2 are male and 2 are female, and none of them are related to eachother they come from 4 different blood lines. if you pair them off and breed them then the offspring will come from one of two bloodlines
in short your breeders are from 4 bloodlines and you produce two bloodlines
A,B,C, and D are each of the 4 frogs.
A B = ab
C D = cd
now you could take ab and cd and breed them together for another bloodline. baisicly any mix and match you can do without inbreeding is a clean bloodline. After a certain number of generations of animals being removed from the original bloodline you could mix them back without defect. Unlike people, darts and many other animals are not effected as severely by inbreeding. There is some research that would suggest that darts can inbreed for up to 10 generations without defect. I would be weary about going to those extremes though.