As Inland Bearded dragons range from semi-arid to desert regions in Australia (most of the eastern inland areas of Australia from southern end of the Northern Territory down nearly the southeast coast and over east into southwestern Queensland and northern New South Wales....lots of temperature variations fall in this large area. Inland Bearded dragons also have one of the largest ranges of the 8 bearded dragon species. (Second Largest range)
In this area, ambient (air) temperature can range anywhere between about 80F to 95F which is pretty much the 80-90F ambient temperature recommended to keep bearded dragons at in captivity (temps higher than 90F can cause over heating if dragons don't have a cooler area to retreat to.). This has nothing to do with surface temp or basking temperature (other than its easier to get a higher basking temp if the air temp is higher and if the cage isn't set up well or too small or doesn't allow air heat to escape...it can heat up the entire cage too much and rob the dragon of a place to cool down in).
Thus, keeping dragons at 100F in one cage and another at 120F (basking temps) isn't going to really prove anything, if both setups have ambient temps in the same range (ie 80-90F). The dragons will just bask longer to keep their internal temperatures in the range they need by absorbing heat. Dragons are also very good at positioning themselves to maximize or minimize heat absorption as well as other adaptations, such as darkening their color, spreading out their bodies to increase surface area, turning their bodies towards the sun, etc.
For any such experiment to know which method is the best method, many factors have to be taken in considering, assuming the dragons used are all healthy and thriving animals (not sickly members of a clutch with bad genes etc). Both cage setups should be the exact same size, have the exact same heating/lighting fixtures and tubes (eliminates variables between different brands and types of bulbs/fixtures) though wattage can vary as needed to obtain the goal temperatures.
Tanks and tank contents should be the same in as many ways as possible. (all should be the same size (for this sort of experiment, tanks should be bigger than minimum... 5'x2' is good and the same height). They should also be built by the same material and style...ideally both should be built by the same person or bought from the same company at the same time (to eliminate changes in manufacturing either by the cage maker or the maker of materials. (for the most part this isn't likely going to be an issue)). Placement of fixtures etc should be same. Basking spot should be of similar material (rocks, logs, etc all absorb and reflect heat in different ways) and color.
What is used to measure temps, should be the same product and model. (If the temp gun is a PE-25 both people should be using this same model...)this eliminates variations in the accuracy of thermometers used.
For temperatures, several spots should be measures...The highest accessible area of the basking spot, a mid point then a low point on the basking area (these should be areas a dragon can put his torso into fully (if only his head and shoulders can fit in the temperature zone, than not really a good indication of what temp the dragon is trying to bask in (midpoint of body should be the target area to take temperatures). Ambient temperatures in the basking area needs to be measured, mid point of cage and far end of cage Temperature outside the cage (ie room temperature) should also be measured.
I also feel at least three temperature readings of all areas should be done. Once in the morning about half hour after lights go on, mid day and around 6pm. Temperature should also be taken at least once during the night to measure nighttime temps.
The tough part is being there (or to have a vidcamera recording the dragon's activities all day long from just before lights on to some time after lights go out. (and this needs a timestamp on it so when reviewed later, the time can be noted when the dragon moves from one spot to another in the tank). Recording how long the dragon stays in the hottest part of the basking spot and at what time he goes there...plus his activities after warming up etc.
Oh and another thing that will have to be monitored and kept the same..is how much food and what kind of food is given. The dragon should be weighed regularly, his food weighed etc. Portions of what type of greens offered in the total food given is also important. (this eliminates variations in diet which could affect how the dragon grows/thrives.) Dragons should be weighed regularly too, especially after bowel movements (to get an idea how much 'weight' is waste matter compared to how much food they get between bowel movements.) On the subject of bowel movements, each one should be recorded as well...more a pain with babies, as they tend to poop more often, but with adults, important to know...and frequency of bowel movements compared to amount of food eaten...may shed light on digestion efficiency in temperature variations.
The goal is to see how dragons utilize temperatures in their environment...do they really use higher temperature basking spots (providing ambient temps don't exceed what they are safe within) or are they just as content to use lower level temperatures and do they thrive better or the same in either areas. Adding too many variables to this experiment will just skew the results (such as using no uvb lights, supplements only, sand substrate or paper towel etc.)
Research in a scientific way is tedious and repetitive with alot of control factors to eliminate unknown variables which can completely affect the results of an experiment. (of course mistakes and unknown variables have lead to great discoveries on their own...but for this debate, it needs to be pretty exact.)
Finally, accurate and consistent record keeping. Don't try to remember the measurements...and write them down later...record everything immediately...and enter that data into a spreadsheet or other format which ensures the data can be easily analyzed later. Make backups! Nothing worse than spending all the money, time and effort on this experiment only to have your results lost in an unexpected Harddrive crash (doesn't hurt to make regular hard copies too and keep your paper notes organized).
-----
PHLdyPayne