Ish, I'm surprised no one answered this post yet. I am not all that qualified to answer it being that I'm relatively new to frog breeding and have bred mostly different chameleon species the past few years but I can help you some. The red eyed eggs should not be sitting in water, that will most likely kill the developing embryos. Just as the eggs were supposed to be, suspended, is how they should be set up. You can place them on a leaf cutting and suspend it above a few inches of water and you will know quickly if the embryos are fertilized because they will rapidly change shape from those white little balls into tadpoles in a week or two if kept around 80 degrees. You can supposedly spray them lightly every day or so, especially when getting close to hatching time to loosen the gelatinous mass. I would keep the egg mass in something humid to keep any eggs from drying out, such as covering the majority of the tank or whatever you have them set up in with plastic wrap or glass. Let me know any progress, updates, and whether or not the eggs are fertile and hatch, etc. To answer your question, mascarino states on their website I believe that many phyllomedusine frogs will sometimes "relieve" themselves of eggs without the use of a male...but since you have males and they are all set up in a rain chamber and you have observed amplexus if I remember your past posts correctly, then you are probably ok.
Kevin