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Hatching Beaded's
caparu
Jan 14, 2006 05:52 PM
Replies (11)
caparu
Jan 14, 2006 05:53 PM
np
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jurassic
Jan 15, 2006 06:39 AM
Very nice, did they incubate on moss the whole duration?
Just curious.
Nice work,
Robert
Sighthunter
Jan 15, 2006 05:56 PM
I have been using acid based substrates for the last six years and have found evidence that alkaline substrate may affect the albumen in the egg negativly.I have not tested reptile Albumin pH but my hunch is that it is 5.0 If the Alkalinity was raised would it kill a viable egg? Here is some Egg Albumin information for what it is worth.
An experimental study has been made of the adsorption of purified egg albumin, from aqueous solution, on collodion membranes. At protein concentrations of 4 to 7 per cent apparent saturation values were obtained which resembled closely the results obtained with gelatin, showing a maximum at pH 5.0 and lower values on either side of this region. Over large ranges of protein concentration, however, the curves for the adsorption from solutions removed in either direction from the isoelectric point exhibited a different shape from the hyperbola obtained in the neighborhood of pH 5.0. The addition of NaCl to solutions on the acid side tended to obliterate the effect of the pH difference; on the alkaline side it greatly increased the adsorption. The adsorption at 25° was about twice as great as that at 1°.
The theory of the swelling of submicroscopic particles, advanced to account for the adsorption behavior of gelatin, is not sufficient to explain the results obtained with egg albumin. It is suggested that the effect is related to alterations in the forces causing the retention of the protein on the membranes.
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HorridumAngeli
Jan 15, 2006 08:24 PM
I am sure you theory has some truth to it. In my experiance Helodermid eggs do best on either pearlite or vemiculite. With the key to success having the humidity just right. You will have to experiment with that your self and see what works best for you.
Take care,
HorridumAngeli
Sighthunter
Jan 15, 2006 10:07 PM
I am assuming that in the wild they are laying their eggs in burrows where mouse feces, urates and organic matter collect. If this is true then the soil may be mostly acidic and this may account for the difficulty in hatching Helioderm eggs. I will indeed expiriment with mine. Life without risk...........
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jurassic
Jan 16, 2006 04:19 AM
Thats very interesting theory. I have hatched several Varanid clutches so far and that was on perlite. But just the same humidity needs to be right as most incubate 200 days. But I would assume they dug there own nest sites as opposed to using a exsisting burrow, they dig for the right temps and condition.None of mine used an existing burrow ever. There wouldnt be any acidic conditions unless the soil was this way.
Glad you posted, nice to hear some new thoughts.
Robert
lateralis
Jan 17, 2006 05:56 PM
Velly interesting....
What albumin was used for this?
I suspect its apples and oranges, or eggs and placentas LOL? Unless these were heloderm eggs from the get go.
Im not sure how constant pH is between species, I mean an egg is an egg, but assuming it is constant than it would really be a matter of finding out how much albumin pH can be affected from outside sources. I would imagine that it is set from the beginning of egg growth and once the egg was shelled either it was right or it was wrong.
If one were to measure the pH of beaded lizard albumin from a successful hatch and included DIEs (dead in egg), malformed, infertile, one might establish a useful baseline of data. In turn one could manipulate the pH of the water used in preparation of inert media like expanded clay, perlite, or vermiculite, and keep it precisely in an acceptable range through direct lowering or raising of the resevoir's pH.
Maybe this has been done already? Mark, Steve, Chris, Bob??
thats my 2cents.
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Cheers
Lateralis
"I would rather be precisely wrong than approximately right"
Marion "Doc" Ford
Sighthunter
Jan 17, 2006 09:32 PM
I am working with a chemist and hobbiest in California to establish a baseline. I have no reptile albumin data as of yet but will have some data soon. I will keep you posted........Bill
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lateralis
Jan 17, 2006 10:07 PM
and it would be an interesting post indeed! Keep us up to date, it seems like it could be useful information.
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Cheers
Lateralis
"I would rather be precisely wrong than approximately right"
Marion "Doc" Ford
Sighthunter
Jan 17, 2006 10:20 PM
I am waiting to hear back from Greg Frey to see if he has any loose data laying around.
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lateralis
Jan 14, 2006 07:11 PM
Nice, congrats I hope to see that one day mi self. Whatcha got there? horridum, exasperatum?
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Cheers
Lateralis
"I would rather be precisely wrong than approximately right"
Marion "Doc" Ford
