With provent a mite I've never had more than one treatment to rid the animal of mites forever. I've only had mites on new animals coming in that are in quarantine and although I treat twice to make sure of no problems, I've never had another incident. I use moss as a substrate, aspen in the cornsnake and false water cobra cages and never had mites in 6 years of keeping....except the two animals I got from someone else and one was from a show.
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2.2~Cornsnakes
3.4.2~Green tree python
3.3.1~ATB
1.1~Sibon Nebulatus
1.0~Eyelash viper
1.1~False Water Cobras
Dogs, cats, horses....
www.franclycac.com