By "positive they are both females" does that mean an educated guess or does that mean you probed them both yourself? Someone else probed them?

Mistakes happen. If there's true locking up (which involves everted hemipenes!) then the obvious thing is that one is definately not a female.

~jen
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"We call them dumb animals, and so they are, for they cannot tell us how they feel, but they do not suffer less because they have no words."
- Anna Sewell (1820-1878)