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Hey! Science types! Dumb question!

meretseger Nov 26, 2003 01:22 PM

I was browsing around the internet and I ran into this, which is a home DNA analyzer for kids. Pretty wacky!

http://shopping.discovery.com/stores/servlet/ProductDisplay?storeId=10000&catalogId=10000&langId=-1&productId=53965&partnumber=691907

So I got this crazy idea wondering if I could use it to compare blood samples from snakes. Like compare how much Eryx colubrinus differs genetically across its range, see how different my Eryx jaculus really are, ect., ect.... Probably a dumb idea. It wouldn't be possible to extract DNA from a small blood sample with the junk in this kit, would it? I know extracting it from strawberries is pretty easy 'cause I read about it in the back of Scientific American.
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Peter: It's OK, I'll handle it. I read a book about something like this.
Brian: Are you sure it was a book? Are you sure it wasn't NOTHING?

Replies (5)

CKing Nov 26, 2003 02:18 PM

It is unlikely that you could achieve your objectives with this toy. It may be better if you contact a systematist and offer him/her your samples and then perhaps you can be credited as being one of the junior authors of a scientific paper for your "work."

meretseger Nov 26, 2003 03:49 PM

I'm not sure I have enough individuals for an actual scientific study that people would accept... For example, I have 3 Egyptian sand boas which are 50% related, and 5 Tanzanian sand boas, which are only from 2 bloodlines. And I'm not even 0 sure of their absolute purity, since some of them are F3 and F3 CB's. So it was kind of just for my own curiosity. On the other hand I DO live 20 minutes from OSU. Hmph, now you have me thinking about getting ahold of a rubber boa and comparing it to my Calabar 'boa'.
I thought that scientists don't do this sort of stuff all the time, I assumed it would be an integral part of taxonomic studies. But I never read about any of it, so maybe they don't do it.
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Peter: It's OK, I'll handle it. I read a book about something like this.
Brian: Are you sure it was a book? Are you sure it wasn't NOTHING?

CKing Nov 27, 2003 12:26 PM

"I'm not sure I have enough individuals for an actual scientific study that people would accept... For example, I have 3 Egyptian sand boas which are 50% related, and 5 Tanzanian sand boas, which are only from 2 bloodlines."

For interspecific molecular comparisons, one individual per species is often enough.

" And I'm not even 0 sure of their absolute purity, since some of them are F3 and F3 CB's."

That could be a problem. Interspecific hybrids could be potentially misleading.

"So it was kind of just for my own curiosity. On the other hand I DO live 20 minutes from OSU. Hmph, now you have me thinking about getting ahold of a rubber boa and comparing it to my Calabar 'boa'. I thought that scientists don't do this sort of stuff all the time, I assumed it would be an integral part
of taxonomic studies. But I never read about any of it, so maybe they don't do it."

Rodriguez-Robles et al. use a Calabar boa and a rosy boa as outgroup to their mtDNA analysis of the rubber boa. Others, like Heise et al. (1995), have found that Rhinophis is closely related to Calabaria. Such a relationship seems surprising but it seems to be corroborated by Wilcox et al. (2002), who show that the lineage leading to Eryx and the lineage leading to Rhinophis are sister groups. Unfortunately, Wilcox et al. did not include Calabaria in their analysis so Heise et al.'s findings have not been directly corroborated by Wilcox et al., but only indirectly corroborated if one assumes a close relationship between Calabaria and Eryx. Scientists could definitely benefit from the donation of additional sources of DNA, especially if these sources are reliable. If you were to write them, many will probably gladly accept your donations.

WW Nov 27, 2003 03:20 AM

You may just about be abel to extract DNA with this toy - problem is, what do you do with it then? I don't think it comes with cloning vectors, PCR machine and automated sequencer...

Cheers,

Wolfgang
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WW Home

meretseger Nov 27, 2003 06:39 AM

Thought I could do something halfway interesting with electrophoresis. Now I'm bummed out. Guess I'll stick with computers...
-----
Peter: It's OK, I'll handle it. I read a book about something like this.
Brian: Are you sure it was a book? Are you sure it wasn't NOTHING?

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