I use either Ed's Fly Meat media (with a sprinkle of baker's yeast, water and aspen wood excelsior stuffed in, or Patrick Nabor's media--Both appear the same. I use the 32 oz. plastic deli cups with the vented lids, which I prefer to those that have one hole and foam. For 50 frogs, I keep about 20 active cultures going at once, starting two new ones every 2-3 days. For renewing the cultures, I use the younger, 14 day old cultures and lace them rather heavily. They are in room light at room temperatures. I haven't had any trouble for a couple of years--no molds, no crashes, although in winter sometimes production gets a bit slow, so I need to keep more cultures. I begin feeding a bit from the cultures when they reach about 10 days old, depending upon the amount of flies in them, and feed less than half from each culture, working down the line from youngest to oldest. Shortages usually occur only because I've neglected to renew them often enough. So far, I've not had molds, mites or other problems. Knock on wood.
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Patty
Pahsimeroi, Idaho

D. auratus blue, auratus Ancon Hill, galactonotus orange, galactonotus yellow, fantasticus, reticulatus, imitator, castaneoticus, azureus, pumilio Bastimentos. P. lugubris, vittatus, terribilis mint green, terribilis orange.

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