With Crested geckos size is no indication of sex, there are females larger than males and vice versa. If two are growing and one is not it may be a sign of them bulling each other around the food dish, but there is no real way to know this unless you watch to make sure they are all eating and poo-ing, a tricky task if they are all in the same tank. The littler one should probably get a home of his/her own.

I, personally, would separate all of them, they know they're sexes before we and an even if you take the little one out, the two left may be a male/male or male/female and cause each other problems. This also allows for easier monitoring of food and such things.

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