I do not breed my females until they are at least 35-40 grams. From a raising stand point, many of mine take a year or a little more to reach that size.

The males will still show an interest in her but she will typically not be interested once gravid. Unless I have a good hunch that she is gravid or if he is overly aggressive after having the opportunities to copulate (actully seeing them do it or constantly seeing the pre-ritual habits through a span of 3 weeks or so)I usually only separate after the first eggs are layed. I do not want any sort of stress on her from having him around. Some people do not separate them at all. To each their own.

My opinion only. Hope this helps.